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Table Of Content New to the PrimerQuest Tool? Primer Design using Gibson Cloning Method High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants Designing PCR Primers Cautions Primer- Definition, Types, Primer Design Online Tools, Uses The number of consecutive Gs and Cs at the 3' end of both the left and right primer. New to the PrimerQuest Tool? Here, we used SADDLE to design a set of 60 primers to detect 56 actionable gene fusions for non-small cell lung cancer (NSCLC) across six genes (ALK, ROS1, RET, NRTK1, NTRK2, and NRTK3). The number of primers are lower than 56 × 2 because the same exon can be fused with multiple partner genes or exons. We detect the fusions in complementary DNA (cDNA) reverse transcribed from RNA, in order to limit the complexity and length of the detection targets. For each fusion of interest, the primer set includes a forward primer (fP) targets the upstream partner gene and a reverse p...
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Emotion Wasatch Canoe BRAND NEW Free Shipping eBay from www.ebay.ca Are you looking for a versatile and reliable canoe for your outdoor adventures? Look no further than the 13 ft Wasatch Canoe. This canoe is perfect for both beginners and experienced paddlers, offering stability, durability, and maneuverability on the water. Whether you're planning a relaxing day on the lake or a thrilling whitewater excursion, the 13 ft Wasatch Canoe is the perfect companion. When it comes to choosing a canoe, there are a few pain points that many people face. Finding a canoe that is stable enough for beginners yet still offers enough performance for more experienced paddlers can be a challenge. Additionally, durability and maneuverability are important factors to consider. The 13 ft Wasatch Canoe addresses all of these pain points, making it an ideal choice for any paddler. The target of the 13 ft Wasatch Canoe is to provide a versatile and reliable option for outdoor enthusiasts. Whether you...
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